Mutation in Shigella flexneri 2a Resulting in Increased Electrophoretic Mobility
نویسندگان
چکیده
منابع مشابه
Dipstick for Rapid Diagnosis of Shigella flexneri 2a in Stool
BACKGROUND Shigellosis or bacillary dysentery, an acute bloody diarrhoea, is a major public health burden in developing countries. In the absence of prompt and appropriate treatment, the infection is often fatal, particularly in young malnourished children. Here, we describe a new diagnostic test for rapid detection, in stool, at the bedside of patients, of Shigella flexneri 2a, the most predom...
متن کاملVaccination against shigellosis with attenuated Shigella flexneri 2a strain SC602.
The Shigella flexneri 2a SC602 vaccine candidate carries deletions of the plasmid-borne virulence gene icsA (mediating intra- and intercellular spread) and the chromosomal locus iuc (encoding aerobactin) (S. Barzu, A. Fontaine, P. J. Sansonetti, and A. Phalipon, Infect. Immun. 64:1190-1196, 1996). Dose selection studies showed that SC602 causes shigellosis in a majority of volunteers when 3 x 1...
متن کاملBiological properties of Shigella flexneri 2A toxin and its serological relationship to Shigella dysenteriae 1 toxin.
A toxin extracted from heat-inactivated, alkaline-treated Shigella flexneri 2a showed biological properties similar to those of Shigella dysenteria 1 toxin. The S, flexneri 2a toxin was lethal to mice, enterotoxic for ileal loops of rabbits, and cytotoxic for HeLa cells. A serological relationship between S. flexneri 2a and S. dysenteriae 1 toxin was shown with cross neutralization tests.
متن کاملMutation in Shigella flexneri resulting in loss of ability to penetrate HeLa cells and loss of glycerol kinase activity.
A colonial variant of a virulent Shigella flexneri 2a has lost both virulence and glycerol kinase activity. It also has several other altered characteristics: lowered ability to oxidize tricarboxylic acid cycle intermediates, increased electrophoretic mobility, and decreased sensitivity to sodium lauryl sulfate. Genetic analysis has revealed that the gene governing glycerol kinase activity in S...
متن کاملEngineered and construction of pDS132::∆virG as suicide vector for targeted gene deletion of virG from Shigella flexneri 2a in order to generation a live attenuated Shigella vaccine
Background & Objective: Shigella are Gram negative bacteria capable of inducing their entry into non-phagocytic cells via secretion of various effector proteins called invasion plasmid antigens (Ipas). The most important of them is VirG protein. Live attenuated Shigella vaccines have indicated promise in inducing protective immune responses in human clinical trials. In current situation, const...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1972
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.5.5.798-802.1972